Publication Title Effect of honey on some biochemical parameters in the brain tissues of Wistar rats exposed to cadmium. Download PDF
Publication Type journal
Publisher Uniport Journal of Engineering and Scientific Research (UJESR)
Publication Authors Diakparomre, Ovovwe; Onyeukwu, Onyeka Benjamin; Asagba, Samuel Ogheneovo
Year Published 2023-10-27
Abstract Cadmium (Cd) toxicity has led to the search for possible ameliorators, which is an interesting area of research currently being explored worldwide. This research investigated the effect of honey on some biochemical parameters in the brain tissues of rats following acute and sub-chronic Cd exposures. Thirty adult Wistar rats were divided into 6 groups (A-F): control, sub-chronic Cd exposure, sub-chronic Cd exposure plus honey, acute Cd exposure, acute Cd exposure plus honey and honey only, respectively. Cadmium chloride (CdCl2) was administered at a dosage of 2 mg/kg body weight by intraperitoneal injection (IP), every two days interval, for a period of 4 weeks (for the sub-chronic study) and a dosage of 4 mg/kg body weight by IP, 12 hours before sacrifice (for the acute study). Honey was administered at a dose of 1 ml/kg body weight orally, once daily for 4 weeks. The study revealed that groups B and D showed significantly higher lipid peroxidation and acid phosphatase (ACP) activity in the brain tissues than that of group A; as well as significantly lower catalase (CAT), superoxide dismutase (SOD), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities. However, administration of honey to Cd-exposed rats ameliorated the Cd-induced changes by causing a reduction in lipid peroxidation and maintaining the activities of these enzymes in the brain tissues within levels significantly similar to that of the control group. This suggests that honey produces an ameliorative effect on Cd-induced injuries in the brain tissues at acute and sub-chronic levels.
Publication Title Microbiological Assessment of WellWater Obtained in Burutu Town, Delta State, Nigeria Download PDF
Publication Type journal
Publisher Continental J. Biological Sciences
Paper Link doi:10.5281/zenodo.3820667
Publication Authors Okiriguo Vivian Ifeoma and Diakparomre Ovovwwe
Year Published 2019-08-30
Abstract This study was carried out to assess the microbial quality of ground water sources (well water) in Burutu town. Five randomly selected wells from five(5) areas of the town were selected and water samples obtained. The samples were subjected to standard methods of analysis. The results showed that the total coliform present in the water samples ranged from 2204 cfu/100ml to 2477 cf/100ml. These values are far above the World Health Organization (WHO) standard of 0cfu/100ml (absence of coliform) for safe potable water. These values may be as a result of poor groundwater source (well) construction, poor handling and lack of maintenance. Individuals who utilize water from these wells for consumption and domestics purposes are at risk of contacting water borne diseases if not properly treated before use.
Publication Title Diagnostic ratios of polyaromatic hydrocarbons for the identification of pollution sources in clams from Okwagbe, Delta State Nigeria Download PDF
Publication Type journal
Publisher Science World Journal
Paper Link https://dx.doi.org/10.4314/swj.v19i2.30
Publication Authors Umudi E.Q., Umudi O.P., Igere O.F. and Diakparomre O.
Year Published 2024-07-05
Abstract Polyaromatic hydrocarbons (PAHs) are pollutants found in the environment. Their sources are anthropogenic and natural. Since clams are filter feeders, they can accumulate (PAHs) in their tissues providing vital tool for pollution source monitoring. This study investigated different pollution sources using diagnostic ratio of PAHs. Clams were collected from Okwagbe River, Delta State, Nigeria and analyzed for their polyaromatic hydrocarbon (PAH) contents. The sixteen (16) priority PAHs, including: 1-methylnaphthalene and 2-methylnaphthalene were determined in flesh and shells using gas chromatography. A mixture of acetone/dichloromethane/n-hexane was used for the Soxhlet extraction. The concentration of mean total PAHs was 2.495 ?g/kg for flesh and 2.156 ?g/kg for shell. The benzo(a)pyrene (B[a]P) of flesh was 0.210 ?g/kg and 0.312 ?g/kg for shells. These valves did not exceed the permissible limit of 6.0 ?g/kg for benzo(a)pyrene and 35.0 ?g/kg for PAHs. The isomeric ratio was used to find the sources of PAHs in flesh and shells. It showed the sources were petrogenic, pyrogenic and wood burning. Clams can be a tool for tracking pollution source identification and improve understanding of human activities on ecosystems.
Publication Title Amelioration of Cadmium-Induced Biochemical Alterations in Heart Tissues of Wistar Rats Using Bee Honey Download PDF
Publication Type journal
Publisher Tropical Journal of Natural Product Research
Paper Link https://www.tjnpr.org
Publication Authors Ovovwe Diakparomre, Onyeka B. Onyeukwu, Samuel O. Asagba
Year Published 2024-08-01
Abstract The hunt for potential remedies for cadmium (Cd) toxicity is an exciting topic of research that is now being investigated globally. This study investigated amelioration of cadmium-induced biochemical alterations in heart tissues of Wistar rats using bee honey. Six groups (1–6) of thirty adult Wistar rats were used for the study: control, sub-chronic Cd exposure, sub-chronic Cd exposure plus honey, acute Cd exposure, acute Cd exposure plus honey, and honey only. For the sub-chronic investigation, Cd chloride (CdCl2) was injected intraperitoneally (IP) at a dose of 2 mg/kg body weight every two days for a period of four weeks, and for the acute trial, a dose of 4 mg/kg body weight was injected IP 12 hours prior to sacrifice. 1 ml/kg of body weight of honey was given orally once every day for four weeks. According to the study, groups 2 and 4 had substantially greater levels of lipid peroxidation and acid phosphatase (ACP) activity in the heart tissues compared to group 1. Catalase (CAT), superoxide dismutase (SOD), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) enzyme activity were also significantly decreased. This study shows that honey treatment significantly ameliorates Cd-induced toxicities in heart tissues by suppressing lipid peroxidation and normalizing antioxidant enzyme activity. Further work should be done to identify specific phytochemical antioxidants and its mode of action in ameliorating Cd-induced toxicities in heart tissues.